Fig 5. Retinoic acid regulates Bcl-2 family protein interactions in MCAO animals.

Immunoprecipitation analysis of Bcl-2 and Bcl-xL to Bad (A and C) or Bax (B and D) in the cerebral cortex from vehicle + middle cerebral artery occlusion (MCAO), retinoic acid (RA) + MCAO, vehicle + sham, and RA + sham animals. Retinoic acid alleviated the increase of Bcl-2/Bad and Bcl-xL/Bad binding and the decrease of Bcl/Bax and Bcl-xL and Bcl-xL/Bax caused by MCAO damage. Each lane represents an individual experimental animal. Densitometric analysis is represented as a ratio of proteins intensity to IgG intensity. Molecular weights (kDa) are depicted at right. Data (n = 5) are represented as mean ± S.E.M. *p < 0.001, **p < 0.01 vs. vehicle + sham animals, #p < 0.01 vs. vehicle + MCAO animals.