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. Author manuscript; available in PMC: 2024 May 16.
Published in final edited form as: J Allergy Clin Immunol. 2019 Jan 17;143(6):2026–2037.e7. doi: 10.1016/j.jaci.2018.11.049

Figure 1: Th1 and Th2 cytokine expression in CD8+ T cells differentiated in IL-2 or IL-2+IL-4 under normoxia or hypoxia.

Figure 1:

CD8+ T cells were differentiated in vitro for 4 days under normoxia (21% O2) or hypoxia (3% O2) in the presence of IL-2 or IL-2+IL-4 followed by SIINFEKL re-stimulation. (A) Representative results (%) of cells staining for IFNγ and IL-13 following SIINFEKL stimulation. (B) Percentages of IL-13 single-positive cells from five independent experiments. (C) Percentages of IFNγ single-positive cells from five independent experiments. (D) Protein levels of IL-13 in 4-day cultures following differentiation in IL-2 or IL-2+IL-4 and activation by SIINFEKL. Results (means±SEM) are from 5 independent experiments. ns: not significant, **p<0.01.