FIGURE 2.

(A) CLUSTAL Omega sequence alignment of the C-terminal region of SNU66 from S. cerevisiae, C. elegans, and human. The bold H with the arrow is the conserved histidine proposed to interact with SNRP-27 M141. (B, C) Cy3 RT-PCR products showing alternative splicing for the alternative 5′ splicing events in mab-10 (B) and Y71H2AM.2 (C) from mixed-stage worm populations. Filled arrows indicate the 5′ss whose usage is known to be promoted by snrp-27(M141T), and the white arrows indicate the 5′ss whose usage is reduced. The small black arrow for Y71H2AM.2 represents the known alternative splice site shown by the doublet on the upper band. The sequences of the alternatively spliced regions are shown above the gels. The percentages that each strain uses the upstream 5′ss with standard deviation (quantified using ImageJ, see Materials and Methods) are shown below the gel images.