TABLE 3.

CRISPR repair oligonucleotides

Strain	Sequence
snu-66 H765 randomized	TGAGTGTGTCTAGTGAATTTTGAAATTTACAACTTTTTTTTTTCAGGTTCNNNGGTCGCAATCCGGGTAAGAAACAGTTcGAaAAACGAGCTAATCGTAAGGA
Prp8 H1573 randomized	ACGGAAAGATCCCGACGCTCAAGATTTCTCTCATTCAAATaTTtCGTGCTNNNTTGTGGCAAAAGATTCACGAGTCAGTAGTTATGGATCTGTGTCAAGTTTT
ubc-22 5′ss swap ΔAA	TATTGAAGATTTATTTCGATCAATCGAGGTAGACGAGGAGGTTAGTTTCTATGTCATGTTTAAATGT∧ACAACGTGGCATTTTCTTTCTAAAAACTTAAGCATTTGACTATTAAAACTGTT
ubc-22 ΔAA	TATTGAAGATTTATTTCGATCAATCGAGGTAGACGAGGAGGTCATGTTCTATGTTAGTTTTAAATGT∧ACAACGTGGCATTTTCTTTCTAAAAACTTAAGCATTTGACTATTAAAACTGTT

NNN indicates that a codon was replaced with randomly incorporated nucleotides at that position. Lower case nucleotides are silent mutations (differ from wt). Underlined are the nucleotides that are mutated. Bold sequences are the restriction enzyme site used. For ubc-22 repair oligos, ^∧ indicates the site of a 2 nt (AA) deletion, 15 nt into the downstream intron, leading to the formation of a BsrGI restriction enzyme cleavage site (TGTACA).