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PCR primers used in screening for CRISPR mutations and tracking alleles
| Strain | Forward primer | Reverse primer |
|---|---|---|
| snrp-27(M141T) | GAGTCGTTACAAAGTGGAGC | TTCGCCATGGTCAAATTCCC |
| snu-66 H765 randomized | GTGGACCAGTTATGCCATTC | CCACTGAGCACAAGATACGG |
| Prp8 H1573 randomized | CTCTGGTGGTCTCCAACTATC | CATAATCTCCCCAACGAAGC |
| ubc-22 5′ss swap | CTCACGGTACGCTGTCATTT | GATGTTCAGTATGTGGGCTTCA |
