Extended Data Fig. 4. SLC7A5 CRISPRi specifically reduces transport of large neutral amino acids.
(a) Comparison of amino acid import rates and inferred export rates in K562 cells growing in RPMI. Import rates are from Fig. 2c, and export rates were calculated by subtracting consumption rates from import rates. (b,c) Additional data related to Fig. 2f,i for amino acids that are not SLC7A5 substrates. (d) Comparison of amino acid import rates in low leucine RPMI and in RPMI for K562 SLC7A5 and non-targeting control (NTC) CRISPRi determined in Fig. 2f. (e) Comparison of intracellular amino acid levels for K562 SLC7A5 and NTC CRISPRi in low leucine RPMI and in RPMI determined in Fig. 2i. (d,e) Asterisks indicate SLC7A5 substrates. Source numerical data are available in source data.