Northern analysis of the ytr transcript. Cells of strain 168 were grown in MM, and RNA samples were prepared at various time points; lanes 1, 2, 3, and 4 contained the RNA prepared at T−1, T0, T1, and T2, respectively. The RNA samples were subjected to a Northern analysis targeting the ytr transcript using the probe (Fig. 1). The position of the ytr transcript is indicated with an arrow. The two strong bands at about 1.5 and 3 kb are due to nonspecific hybridization to rRNAs.