FIG. 4.

Detection of biotinylated polypeptides in the protein extracts of wild-type and accA mutant strains. Proteins, biotinylated molecular mass standards (MW), and purified M. xanthus propionyl-CoA carboxylase (PCC-α) were separated by SDS–12% PAGE (A) or SDS–8% PAGE (B) and probed with streptavidin-linked horseradish peroxidase. The protein extracts were prepared from cultures at the exponential growth phase (E), at stationary phase (S), and during development (D).