TABLE 1.
Fraction | Acetyl-CoA carboxylase
|
Propionyl-CoA carboxylase
|
||
---|---|---|---|---|
Total activity (mU) | Sp act (mU/mg of protein) | Total activity (mU) | Sp act (mU/mg of protein) | |
Wild type | 22.3 | 1.0 | 69.0 | 3.1 |
accA mutant | 12.3 | 0.6 | 60.9 | 3.0 |
The acetyl-CoA and propionyl-CoA carboxylase activities were determined with acetyl-CoA and propionyl-CoA as substrates from the rate of malonyl-CoA and methylmalonyl-CoA formations, respectively. The total and specific activities were expressed as means of triplicate enzyme assays.