Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 11;43(10):1965–1989. doi: 10.1038/s44318-024-00086-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A, B) TF motifs are significantly enriched in CO/OC/PO categories of ATAC-seq peaks during the transition from SL-to-2iL (A) and 2iL-to-SL (B). The motifs for TFs are indicated on the right of the heatmap. *p < 1e−30. P-value was calculated by hypergeometric enrichment calculations from Homer software. (C) RT-qPCR testing siRNA knockdown efficiencies for Tead2, Tead4, Tcfcp2l1, Nr5a2, and Esrrb during SL-to-2iL transition. Cells were treated with specific siRNAs for every 3 days along with control siRNA. Data are shown as the mean ± SD. Indicated significances are tested using Student’s t-test analyses (***p < 0.001). n = 3 biological replicates. (D) Representative images of cells transfected with siNC (negative control) and siRNAs for Tead2, Tead4, Tcfcp2l1, Nr5a2, and Esrrb during the SL-to-2iL process. Scale bar, 100 μm. (E) Representative images of AP staining of cells transfected with siNC (negative control) and siRNAs for Tead2, Tead4, Tcfcp2l1, Nr5a2, and Esrrb during SL-to-2iL process. Scale bar, 100 μm. (F) The percentage of clones with abnormal morphology on day 3 of SL-to-2iL transition in (E). Data are shown as mean ± SD of three independent fields of view. (G) Number of cells transfected with siNC (negative control) and siRNAs of Tead2, Tead4, Tcfcp2l1, Nr5a2, and Esrrb. Cells were grown for 3 days during the SL-to-2iL process. Data are presented as the mean ± SD of three independent wells. Indicated significances are tested using Student’s t-test analyses (*p < 0.05, ***p < 0.001). n = 3 biological replicates. Source data are available online for this figure.