Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Apr 11;43(10):1965–1989. doi: 10.1038/s44318-024-00086-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

PMC Copyright notice

(A) Scatter plot displaying the changes of TEAD2-mediated EP interactions in the normalized interaction frequency (log₂ fold change) between wild-type cells and Tead2-knockout cells collected at day 6 during the SL-to-2iL transition. (B) Changes in the normalized interaction frequency (log₂ fold change) in cells at day 6 during the SL-to-2iL transition upon Tead2-knockout in the following three different categories: all interactions (n = 701,601), interactions unrelated to TEAD2 binding sites (n = 84,504), and TEAD2-mediated EP interactions (n = 16,683). The centerline indicates the median value, while the box and whiskers represent the interquartile range (IQR) and 1.5 × IQR, respectively. ***p < 0.001. P-value was calculated by using Mann–Whitney U test. (C) Bar plot showing the numbers of the differential TEAD2-mediated EP interactions after Tead2-knockout. (D) Normalized values of the differential TEAD2-mediated EP interactions frequency (Increased interactions, n = 1438; Decreased interactions, n = 3391). The centerline indicates the median value, while the box and whiskers represent the interquartile range (IQR) and 1.5 × IQR, respectively. P-value was calculated by using Mann–Whitney U test. (E) Heatmaps showing APA of differential TEAD2-mediated EP interactions in both wild-type and Tead2-knockout cells at day 0 and day 6 of the transition. (F) Venn diagrams showing overlap between the genes with decreased EP interactions and genes in cluster 5. P-values were calculated with the hypergeometric distribution test. (G) GO categories of overlapping genes shown in (F). (H) Representative genomic locus showing the binding of TEAD2 and H3K27ac, and decreased chromatin interactions after Tead2-knockout. B4galt6 promoter is highlighted with red-shaded rectangles, and its associated enhancers are highlighted with green-shaded rectangles.