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. 2024 Apr 10;43(10):2062–2085. doi: 10.1038/s44318-024-00087-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the data associated with this article, unless otherwise stated in a credit line to the data, but does not extend to the graphical or creative elements of illustrations, charts, or figures. This waiver removes legal barriers to the re-use and mining of research data. According to standard scholarly practice, it is recommended to provide appropriate citation and attribution whenever technically possible.

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Figure EV5 — (A) Reconstruction obtained from γ-TuRC-focused refinement of γ-TuRCs capping Paclitaxel-stabilised microtubules which were (top) or were not (bottom) subjected to the shortening procedure during purification. A model for the γ-TuRC was generated for each reconstruction separately by spoke-wise rigid body docking. A high confidence threshold is used to highlight the fit at the level of γ-tubulin. Spoke numbering is indicated. Colouring as in Fig. 2A. (B–E) Euclidian translation distance (B), downward change in helical pitch (C), translation towards the MT axis (D) and rotation around the MT axis (E) required to convert the models to the hypothetical, fully closed γ-TuRC for each spoke. Parameters measured from the centre of mass of γ-tubulin. (F) Reconstructions obtained from MT-dominated refinements of γ-TuRC-capped Paclitaxel-stabilised MTs which were (white) or were not (purple) subjected to the shortening procedure during purification. Density for spokes in the background was hidden for visual clarity. Colouring scheme is indicated. (G) Coordinates of the centre of mass for the first α-tubulin in each protofilament in the plane orthogonal to the MT axis for the regular 13 protofilament lattice (grey), the Paclitaxel-stabilised γ-TuRC-capped MT where shortening was (dark blue) or was not (green) applied during purification. Individual protofilaments could not be fitted at spokes 4 to 6 for the condition without shortening due to lower density quality. Protofilaments are numbered by the respective spokes in the γ-TuRC. The MT axis is placed on the origin, (0,0). Colouring scheme is indicated. Source data are available online for this figure.