Figure 4. CAMSAP2 specifically recognises the minus end of γ-TuRC-capped MTs nucleated through the RanGTP pathway.

(A) Exemplary MTs nucleated through the RanGTP pathway in X. laevis egg extract that show colocalisation of the γ-TuRC (yellow) and GFP-CAMSAP2 (red) at the minus end of MTs (blue), as judged by fluorescence microscopy. Scale bar represents 1 μm. (B) Normalised average intensity of CAMSAP2 (red), γ-TuRC (yellow) and MT signals (blue) along the length of γ-TuRC-capped MTs (n = 69). Only MTs longer than 5 μm were considered; data were combined from three biological replicates. (C) Average intensity of CAMSAP2 signal within 0.6 μm of the γ-TuRC signal peak (n = 552 from 69 MTs, of which 28, 19 and 22 for each respective replicate) or further away (n = 823 from 69 MTs). Intensities were normalised to the mean CAMSAP2 intensity on the MT lattice for each individual replicate. p = 3.2 × 10⁻⁶⁸ Dots indicate mean values of the three biological replicates. ***p < 0.001 by a one-tailed Welch’s t-test. Data in (B) and (C) shown as mean with 95% confidence interval for all individual data points. Source data are available online for this figure.