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. 2024 Apr 8;16(5):1091–1114. doi: 10.1038/s44321-024-00062-w

Figure EV3. Both mRNA and protein level of R/R decrease in aging flies induced by PIL complex dysregulation.

Figure EV3

(A) Relative mRNA abundance in whole fly extracts of M/M and R/R male flies with its corresponding K/K control in week 1 and week 3; n = 12 flies/genotype from 3 independent technical replicates. (B) Relative mRNA abundance in whole fly extracts of M/M and R/R female flies with its corresponding K/K control in week 1 and week 3; n = 12 flies/genotype from three independent technical replicates. (C) Representative western blotting of whole fly extracts from M/M and R/R flies with its corresponding K/K control in week 1 and week 3; n = 5 flies/genotype. (D) Co-immunoprecipitation to examine the association of FLAG-hINSC (WT and M70R) with MYC-LGN (left) or HA-PAR3 (right) in SH-SY5Y cells.