Fig. 4. The HOPS complex is required for the activation of TOR pathway.
a Rapamycin sensitivity examined by spot assay. C. albicans strains were serial 5-fold diluted, spotted on YPD agar containing rapamycin and cultured at 30 °C for 48 h. b The phosphorylation of RPS6 protein (P-S6) examined by western blotting. The representative images shown are from 3 biological repeats. c The hyphal morphology of C. albicans strains on solid medium. Three biological replicates were carried out. One of the representative images are shown. Width (W) and depth (D) (mm) were quantified by Image J. n = 5 colonies. Scale bars = 1 mm. d Relative expression of GTR1 and TOR1 in GTR1OE-vam6Δ/Δ mutants compared to SC5314 examined by quantitative real-time RT-PCR. n = 3 samples. e Rapamycin sensitivity examined by spot assay. f The ratio of germination in C. albicans cultured in hyphal-inducing liquid media at 37 °C for 0.5 h. The ratio of germinated strains to all the strains observed in the microscopic field (approximately 50–100 fungal cells in each field under the 40× objective) was considered as the ratio of germination. n = 3 fields. g The hyphal length quantified by Image J. n = 50 hyphae. h The hyphal morphology of C. albicans strains on solid medium. Three biological replicates were carried out. One of the representative images are shown. Width (W) and depth (D) (mm) were quantified by Image J. Scale bars = 1 mm. n = 3 colonies. Data were presented as mean ± SD (c, d, f, h). Box plots indicate median (middle line), 25th, 75th percentile (box) and minimum and maximum value as well as outliers (single points) (g). Two-tailed unpaired t-test (c, d, f, g, h). Source data are provided as a Source Data file.