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Schematic representation of the AML cell differentiation protocol
PBMCs are isolated from heparinized blood and are either used directly or undergo monocyte isolation prior to AML cell differentiation. Cells can be cultured without the Infasurf, GM-CSF, TGF-β and IL-10 (ALL cocktail) to generate untreated (UT) MDMs or with the ALL cocktail to generate AML cells. Created with Biorender.com.
