| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Human CD64-FITC (1:50 dilution) | BioLegend | Cat# 305006 RRID:AB_314490 |
| Mouse IgG1 κ isotype control-FITC (1:50 dilution) | BioLegend | Cat# 400108 RRID:AB_396090 |
| Human CD163-PE (1:50 dilution) | eBioscience | Cat# 12-1639-42 RRID:AB_1963570 |
| Mouse IgG1 κ isotype control-PE (1:50 dilution) | eBioscience | Cat# 12-4714-82 RRID:AB_470060 |
| Human CD206-BV421 (1:50 dilution) | BioLegend | Cat# 321126 RRID:AB_2563839 |
| Mouse IgG1 κ isotype control-BV421 (1:50 dilution) | BioLegend | Cat# 400158 RRID:AB_11150232 |
| Human MARCO-PE (1:50 dilution) | eBioscience | Cat# 12-5447-42 RRID: AB_2762430 |
| Mouse IgG3 isotype control-PE (1:50 dilution) | eBioscience | Cat#12-4742-42 RRID: AB_10733011 |
| Human CD36-PE (1:50 dilution) | BD Biosciences | Cat# 555455 RRID: AB_395848 |
| Mouse IgM κ isotype control-PE (1:50 dilution) | BD Biosciences | Cat# 555584 RRID: AB_395960 |
| Human TruStain FcX (Fc receptor blocking solution) (1:50 dilution) | BioLegend | Cat#422302 RRID: AB_2818986 |
| Cell staining buffer | BioLegend | Cat#420201 |
| Anti-human CD14-PerCP-Cy5.5 (1:20 dilution) | Calibre Scientific | Cat# CAPR-103465 |
| Human MARCO APC (1:20 dilution) | eBioscience | Cat# 17-5447-42 RRID: AB_2762440 |
| Mouse IgG3, κ isotype control- Alexa Fluor 647 (1:20 dilution) | BioLegend | Cat# 401322 RRID: AB_10683446 |
| 7-AAD viability staining solution | BioLegend | Cat#420404 |
| Biological samples | ||
| Heparinized whole blood | This manuscript | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| GMP recombinant human GM-CSF (carrier-free) | BioLegend BD Biosciences |
Cat# 572914 Cat# 550068 |
| Recombinant human TGF-β1 (carrier-free) | BioLegend | Cat# 781804 |
| Recombinant human IL-10 | BD Biosciences | Cat# 554611 |
| Infasurf/CUROSURF | ONY Biotech, Inc. and Chiesi Farmaceutici, S.p.A, hospital systems | N/A |
| RPMI 1640 medium, GlutaMAX supplement | Gibco/Invitrogen, Thermo Fisher Scientific | Cat# 11875-093; Cat# 61870044 |
| Human AB serum | Sigma-Aldrich | Cat# H4522 |
| Lymphoprep | STEMCELL Technologies | Cat# 07851 |
| Ficoll-Paque PLUS | GE Healthcare/Sigma | Cat# 17-1440-03 |
| Heparin sodium, 1,000 U/mL | Nationwide Medical Surgical/Sagent Pharmaceuticals | Cat #25021040010 |
| Gibco cell dissociation buffer | Thermo Fisher Scientific | Cat# 13-150-016 |
| 0.9% sodium chloride, irrigation | Abbott Laboratories | |
| DPBS 1x, no calcium, no magnesium | Thermo Fisher Scientific | Cat# 14190169 |
| Critical commercial assays | ||
| Classical monocyte isolation kit | Miltenyi | Cat# 130-117-337 |
| EasySep human monocyte isolation kit | STEMCELL technologies | Cat# 19359 |
| Direct-zol RNA microprep kit | Zymo Research | Cat# R2062 |
| High-capacity RNA-to-cDNA kit | Applied Biosystems | Cat# 4387406 |
| TaqMan fast universal PCR master mix (2X), no AmpErase UNG | Thermo Fisher Scientific | Cat# 4352042 |
| Oligonucleotides | ||
| GUSB | Thermo Fisher Scientific | Cat# 1702016 |
| SPI1 | Thermo Fisher Scientific | Cat# Hs02786711_m1 |
| MRC1 | Thermo Fisher Scientific | Cat# Hs00267207_m1 |
| PPARG | Thermo Fisher Scientific | Cat# Hs01115513_m1 |
| ACTB | Thermo Fisher Scientific | Cat# Hs01060665_g1 |
| Software and algorithms | ||
| Prism software 9.3.1 | GraphPad by Dotmatics | N/A |
| FlowJo 10.8.1 | BD Biosciences | N/A |
| ViiA 7 software | Thermo Fisher Scientific | N/A |
| BioRender | Institution subscription | AV26P8RJCI, II26NWLUOR, FE26NWLKOB |
| ImageJ | National Institutes of Health | ImageJ-win64 |
| Other | ||
| LS columns | Miltenyi | Cat# 130-042-401 |
| Nunc 96-well polystyrene conical bottom MicroWell plates | Thermo Fisher Scientific | Cat# 249570 |
| QuadroMACS separator | Miltenyi | Cat# 130-090-976 |
| EasySep magnet | STEMCELL Technologies | Cat# 18000 |
| 50 mL Falcon tubes | Corning | Cat# 352098 |
| 15 mL Falcon tubes | Thomas Scientific | Cat# 12-565-269 |
| BD LSRFortessa X-20 | BD Biosciences | N/A |
| BD FACSymphony | BD Biosciences | N/A |
| ViiA 7 real-time PCR system | Thermo Fisher Scientific | N/A |
| Applied Biosystems 7500 real-time PCR system | Applied Biosystems | N/A |
| Beckman Allegra 6R centrifuge | Beckman Coulter | N/A |
| Beckman Allegra X-15R centrifuge | Beckman Coulter | N/A |
| Motic AE2000 inverted biological microscope with Moticam | Motic | N/A |
| Glass tubes for serum | Corning (Fisher) | Cat# 8422-50 (05-588-5B) |
| Teflon jar 2 fL. Oz/60 mL (holds 7–8 mL volume) | Savillex | Cat# 100-0060-01 |
| Teflon wells lids, 53 mm Closure | Savillex | Cat# 600-053-01 |
| Teflon jar 4 fL. Oz/120 mL (holds 13–15 mL volume) | Savillex | Cat# 100-0120-01 |
| Teflon well lids, 70 mm closure | Savillex | Cat# 600-070-01 |
| Steriflip-GP sterile centrifuge tube top filter unit | Millipore/Sigma | Cat# SCGP00525 |
| Falcon round-bottom polypropylene tubes | Falcon | Cat# 352063 |
| Falcon round-bottom polystyrene tubes with cell strainer snap cap, 5 mL | Falcon | Cat# 352235 |
| Falcon 96-well, non-treated, U-shaped-bottom microplate | Falcon | Cat# 351177 |
Reagents and supplies for monocyte collection and isolation.