Figure 4.

Associations of health outcomes with DNAm CRP from elastic net regression and assay-measured CRP

Linear and logistic regression models (two-sided) were used to test for cross-sectional associations of DNAm and assay-measured (i.e., phenotypic) CRP with cardiometabolic, lifestyle, and self-report disease variables at wave 2 of the LBC1936 (N ≤ 756). Cox proportional hazard models tested for associations between CRP (assay measured or DNAm) derived at wave 2 and time to death due to all-cause mortality. Here, only DNAm CRP from elastic net regression was utilized, given that it was deemed the best-performing method in correlation analysis and incremental R² modeling. Association tests using DNAm CRP from other prediction strategies and a polygenic score for CRP are shown in Figures S3–S8. CRP, C-reactive protein; DCCT, Diabetes Control and Complications Trial; DNAm, DNA methylation; HDL, high-density lipoprotein; Thrombopl., thromboplastin.