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Western blot showing α subunits formed by JM109(DE3) carrying pDTG141 derivatives with the amino acid substitutions indicated below. A monoclonal antibody specific for the α subunit of NDO was used as described in Materials and Methods. Lanes: M, prestained molecular mass markers (Bio-Rad Laboratories); 1, purified wild-type NDO (2 μg); 2, wild-type NDO (pDTG141); 3, vector control (pT7-5); 4, F352G; 5, F352A; 6, F352I; 7, F352T; 8, F352W; 9, F352Y.
