TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsa | Source or reference(s) |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| DH5α | Δ(lacZYA-argF)U169 hsdR17 relA1 supE44 endA1 recA1 thi gyrA96 φ80dlacZΔM15 | Life Technologies, Gaithersburg, Md. |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB) mcrA (F′ traD36 proAB+ lacIqZΔM15) | 55 |
| JM109(DE3) | endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB) mcrA (F′ traD36 proAB+ lacIqZΔM15) λ(DE3) | Promega Corp., Madison, Wis. |
| ES1301 mutS | KmrlacZ53 mutS201::Tn5 thyA36 rha-5 metB1 deoC IN(rrnD-rrnE) | Promega Corp. |
| Pseudomonas putida | ||
| UV4 | Toluene-degrading strain | 6 |
| F1 | Toluene-degrading strain | 17, 18 |
| Sphingomonas yanoikuyae B8/36 | cis-Biphenyl dihydrodiol dehydrogenase mutant of strain B1 | 19, 32 |
| Plasmids | ||
| pDTG141 | Apr, nahAaAbAcAd (encoding the naphthalene dioxygenase components reductaseNAP, ferredoxinNAP, and large and small subunits of the oxygenase, respectively) under the control of the T7 promoter of pT7-5 | 52 |
| pT7-5 | Apr, T7 expression vector | 54 |
| pMASTER-1 | Tcr Aps, pALTER-1 carrying the KpnI-XbaI fragment of pDTG141 (nahAc′Ad) | 39 |
| pDTG511 | Kmr, pKT230 containing todD gene encoding cis-toluene dihydrodiol dehydrogenase from P. putida F1 | 56 |
| pDTG512 | Kmr, pKT230 containing truncated todD gene from P. putida F1 | 56 |
| pKT230 | Kmr Smr, broad-host-range cloning vector | 4 |
a
Kmr, kanamycin resistance; Apr, ampicillin resistance; Tcr, tetracycline resistance, Smr, streptomycin resistance.