TABLE 3.
Rates of product formation by wild-type and mutant NDO enzymes
| NDO enzyme | Naphthalene cis-dihydrodiol formationa
|
Biphenyl cis-2,3-dihydrodiol formationa
|
Biphenyl cis-3,4-dihydrodiol formationa
|
|||
|---|---|---|---|---|---|---|
| sp act (nmol/min/mg) | Relative activity (%) | sp act (nmol/min/mg) | Relative activity (%) | sp act (nmol/min/mg) | Relative activity (%) | |
| Wild type | 20.4 | 100 | 4.81 | 100 | 0.95 | 100 |
| F352G | 7.4 | 37 | <0.05 | <1 | <0.25 | <26 |
| F352A | 9.9 | 49 | 0.07 | 1 | 0.25 | 26 |
| F352T | 15.6 | 77 | 0.14 | 3 | 0.73 | 77 |
| F352V | 16.9 | 83 | 0.15 | 3 | 0.94 | 99 |
| F352I | 16.5 | 81 | 0.16 | 3 | 0.74 | 78 |
| F352L | 19.5 | 96 | 0.21 | 4 | 0.82 | 86 |
a
The formation of naphthalene and biphenyl cis-dihydrodiols was measured spectrophotometrically as described in Materials and Methods.