TABLE 4.

Enantiomeric composition of products formed by wild-type and mutant NDO enzymes

NDO enzyme	Enantiomeric compositiona of:
	Naphthalene cis-1,2-dihydrodiol	Biphenyl cis-2,3-dihydrodiol	Biphenyl cis-3,4-dihydrodiol
Wild type	>99% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	>98% (+)-(3R,4S)
F352G	98% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	60% (+)-(3R,4S)
F352A	96% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	65% (+)-(3R,4S)
F352T	93% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	60% (−)-(3S,4R)
F352V	92% (+)-(1R,2S)-	NDb	77% (−)-(3S,4R)
F352I	94% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	53% (+)-(3R,4S)
F352L	96% (+)-(1R,2S)-	>95% (+)-(2R,3S)-	70% (+)-(3R,4S)

^aEnantiomeric composition was determined by chiral stationary-phase HPLC as described in Materials and Methods. 

^bND, not determined.