TABLE 2.
Relevant features of plasmids used or constructed during this work
| Plasmid | Feature (reference) |
|---|---|
| pUC18 | Versatile E. coli vector (36) |
| pIJ2926 | pUC18 derivative with modified polylinker (17) |
| pKK2006 | pUC18 carrying tsr for selection in Streptomyces |
| pKK974 | 1.4-kb aac(3)IV-containing BamHI fragment in pIJ2925 (A. J. Dharmatilake, personal communication) |
| pKK1333 | pIJ2926 derivative containing 10-kb BamHI fragment from pKK1336 |
| pKK1334 | pIJ2926 derivative containing 10-kb BamHI fragment from pKK1337 |
| pKK1335 | pIJ2926 derivative containing 10-kb BamHI fragment from pKK1338 |
| pKK1336 | pXE4 derivative with 10-kb BamHI insert containing ssfR and ssgA |
| pKK1337 | pXE4 derivative with 10-kb BamHI insert containing ssfR and ssgA |
| pKK1338 | pXE4 derivative with 10-kb BamHI insert containing ssfR and ssgA |
| pKK1343 | pKK1333 derivative in which one BamHI site has been removed using KpnI digestion and religation |
| pKK1349 | pIJ2926 derivative containing 3.6-kb HincII insert from pKK1343 |
| pKK1351 | pIJ2926 derivative containing mutated version of 3.6-kb HincII insert |
| pKK1355 | pXE4 derivative containing 3.9-kb BglII insert from pKK1349 |
| pKK1356 | pXE4 derivative containing 3.9-kb BglII insert from pKK1351 |
| pKK1358 | pIJ2926 derivative containing 1.6-kb StyI insert from pKK1349 |
| pKK1359 | pIJ2926 derivative containing 1.2-kb MspA1I insert from pKK1349 |
| pKK1361 | pKK2006 derivative containing 3.9-kb BglII insert from pKK1349 |
| pKK1362 | pKK2006 derivative containing 3.9-kb BglII insert from pKK1351 |
| pKK1367 | pIJ2926 derivative containing 2.5-kb PstI insert from pKK1343 ExoIII deletion fraction 10-4 (Fig. 1) |
| pKK1368 | pKK1343 ExoIII deletion fraction 13-1 derivative in which 5.7-kb ClaI-EcoRI fragment has been removed (Fig. 1) |
| pKK1369 | pKK1367 derivative in which 160-bp ApaI fragment has been removed |
| pKK1370 | pKK1369-based construct containing, within ssgR, a 1.4-kb BamHI fragment containing the aac(3)IV apramycin resistance marker, excised from pKK974 |
| pKK1371 | pKK1368-based construct containing, within ssgA, a 1.4-kb BamHI fragment containing the aac(3)IV apramycin resistance marker, excised from pKK974 |
| pKK1372 | pKK2006 derivative containing 1.5-kb BglII insert from pKK1358 |
| pKK1373 | pKK2006 derivative containing 1.4-kb BglII insert from pKK1368 |
| pKK1374 | pKK2006 derivative containing 3.9-kb BglII insert from pKK1370 |
| pKK1375 | pKK2006 derivative containing 2.8-kb BglII insert from pKK1371 |
| pKK1376 | pKK1343 ExoIII deletion fraction 15-1 derivative in which 5.7-kb ClaI-EcoRI fragment has been removed (Fig. 1) |
| pKK1377 | pKK2006 derivative containing 1.2-kb BglII insert from pKK1376 |
| pKK1385 | pKK1367 derivative in which EcoRI-BamHI fragment has been removed |
| pKK1391 | pKK1367-based construct in which 600-bp SstI fragment has been replaced with 1.4-kb aac(3)IV apramycin resistance marker excised from pKK974 |
| pKK1392 | pKK2006 derivative containing 3.4-kb BglII insert from pKK1391 |