FIGURE 6.

RT‐PCR reveals c.1986_1987insCC leads to complete null ITGA2B expression. Gel image of ladder (left) and the resulting RT‐PCR products are presented. ACTB housekeeper amplification was confirmed in a sex‐ and age‐matched clinically healthy cat (lane 1) and affected patient (lane 2) (bands between 650 and 500 bp marker). ITGA2B expression up‐ and downstream of the variant position (bands between 300‐200 bp and 200‐100 bp marker, respectively) was detected in the control cat (lane 4 and lane 7, respectively), but not in the affected cat (lane 5 and lane 8, respectively). Negative‐control samples for the ACTB and 5′ and 3′ ITGA2B RT‐PCR reactions are displayed (lane 3, lane 6, and lane 9, respectively). Original gel image is provided in Figure S1. ACTB, beta (β)‐actin; bp, base pair; ITGA2B, integrin alpha‐IIb.