TABLE 1.

Sanger sequencing genotyping & RT‐PCR primer specifications.

Primer name	Primer sequence	GC%	Exon Target(s)	Tm (°C)	Product size
PCR
ITGA2B_Genotyping_F	GCGGGTGCTACTGCTGAAT	57.9	18	56	676
ITGA2B_Genotyping_R	TGAAAAGGAGTTTGGAGCTGA	42.9
RT‐PCR
ACTB_F	ATGAGGCCCAGAGCAAGAG	57.9	2–3	61	510
ACTB_R	CTTCTCCAGGGAGGACGAG	63.2
ITGA2B_Upstream_F	CACTGAATCCTGCTGTGAAGA	47.6	15‐18	61	255
ITGA2B_Upstream_R	CTCAGCTTGTCCCGGAAGT	57.9
ITGA2B_Downstream_F	GCGGAGAGGATGATCTGTGT	55	19‐20	63	129
ITGA2B_Downstream_R	CCTCATGTAGTGGGCACCTG	60

Abbreviations: ACTB, beta (β)‐actin; GC%, guanine‐cytosine percentage; ITGA2B, integrin alpha‐IIb; Tm, melting temperature.