TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| GS162 | E. coli K-12 ΔlacU169 | This laboratory |
| GS244 | GS162 derivative ΔmetR::Mu | This laboratory |
| GS972 | GS162 derivative carrying chromosomal operator constitutive mutation in metR promoter | This laboratory |
| GS1040 | GS162 derivative carrying chromosomal E261K rpoA allele | This laboratory |
| GS1106 | GS162 derivative carrying chromosomal rpoA112 allele | This study |
| BL21(DE3) | E. coli λDE3, which encodes T7 RNAP from the lacUV5 promoter | Novagen |
| Plasmids | ||
| pREIIα (and derivatives) | ori-pBR322; lppP-′lacPUV5-rpoA (and derivatives carrying N268D, L270H, alanine substitutions at residues 275 to 301 and 303 to 329, or N-terminal Strep tag) | 3, 10, 18, 53; This study |
| pHTf1α (and derivatives) | ori-pBR322; ori-f1; lppP-′lacPUV5-rpoA (and derivatives carrying alanine substitutions at residues 255 to 271, 273, or 302) | 10, 42 |
| pHTT7f1-NHα (and derivatives) | ori-pBR322; ori-f1; φ10P-rpoA with N-terminal His6 tag (and derivatives carrying R265A, N268D, L270H, or G296A substitutions, or ΔCTD) | 41; this study |
| pT7-Strepα (and derivatives) | ori-pBR322; ori-f1; φ10P-rpoA with N-terminal Strep tag (and derivatives carrying an R45A substitution, ΔCTD, or an R45A substitution plus ΔCTD) | This study |
| pMKSe2 | ori-pBR322; lacP-rpoB | 38 |
| pT7β′ | ori-pBR322; φ10P-rpoC | 56 |
| pRLG593 | ori-pBR322; lacPUV5 | 35 |
| pGS395 | ori-pDF41; tacP-metR | This laboratory |