Table 3:
Culture model, study | DTG dosage (μM)* | N | Results |
---|---|---|---|
Murine P19C5 pluripotent stem cells and human embryonic stem cells H944 | 0·25, 0·5, 1, 2, 4 | 46–48 aggregates per condition for morphogenesis | DTG was associated with impaired stem cell morphogenesis and changes to developmental regulator genes in a dose-dependent manner in both P19C5 and H9 cells. |
CA1S human embryonic stem cells45 | 8·32 | 5 replicates | DTG was associated with reduced expression of pluripotency markers in CA1S cells. |
H9 human embryonic stem cells45 | 8·32 | 6 replicates | DTG was associated with increased rates of apoptosis in H9 cells. |
Sprague Dawley GD9 embryo culture42 | 12·6 | 16 embryos | DTG did not affect embryo lethality, visceral yolk sac, somite number, or embryo size. |
22·2 | 16 embryos | ||
Zebrafish embryo culture49 | 100 | 2–4 experimental replicates | DTG was associated with developmental toxicity post-fertilization. |
Cmax for DTG in non-pregnant adults is reported as 701–1156 μM.103