TABLE 2.
Percentages of germinated spores with ring-shaped nucleoidsa
| Strainb | Germinated spores with ring-shaped nucleoids (%) |
|---|---|
| B. megateriumQMB1551 (wt) | 86 |
| B. megaterium PS1551 (gpr) | 65 |
| B. subtilis PS832 (wt) | 72 |
| B. subtilis PS1029 (gpr) | 85 |
| B. subtilis PS260 (α−) | 22 |
| B. subtilis PS223 (β−) | 14 |
| B. subtilis PS578 (α− β−) | 3 |
| B. subtilis PS1450 (α− β− pSspCwt) | 58 |
| B. subtilis PS1465 (α− β− pSspCAla) | 22 |
a
Spores of various strains were germinated for 2 min, fixed, and stained with DAPI as described in Materials and Methods. The presence of ring-shaped nucleoids was scored in random fields of a fluorescence microscope; 300 spores containing nucleoids stained with DAPI were counted for each strain.
b
wt, wild type.