Table 3.
Summary of attempts to generate primary Glossina morsitans morsitans cell cultures in the present study
| Culture | Tissue (stage) | Medium1 | Outcome |
|---|---|---|---|
| 1 | Larva (L3) | L-15 | Metabolism but no growth, combined with #2, 4 and 6 on day 189, discarded on day 252 |
| 2 | Larva (L3) | L-15B | Metabolism but no growth, combined with #1, 4 and 6 on day 189, discarded on day 252 |
| 3 | Larva (L3) | Schneider’s | Small patch of growing cells seen on day 79, three patches seen on day 99; combined with #5 on day 189, no further growth, discarded on day 252 |
| 4 | Larva (L2) | H-Lac | Metabolism but no growth, combined with #1, 2, and 6 on day 189, discarded on day 252 |
| 5 | Larva (L2) | Schneider’s | Some contracting cells seen on day 79, twitching cell clumps seen on day 125, combined with #3 on day 189, no further growth, discarded on day 252 |
| 6 | Larva (L2) | L-15B | Metabolism but no growth, combined with #1, 4 and 6 on day 189, discarded on day 252 |
| 7 | Two larvae (L1) | L-15, L-15B from day 7 | Metabolism but no growth, discarded on day 562 |
| 8 | Adult tissues | L-15 | Slow growth, 1st passage on day 437. Developed into cell line GMA/LULS61 |
1Medium formulations are described in Methods