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. 2000 Oct;182(19):5563–5571. doi: 10.1128/jb.182.19.5563-5571.2000

FIG. 2.

FIG. 2

Suppression of JB1A8v invasin motility phenotypes by pJB69. (A) Western analysis of invasin expression by JB41v(pTM100), JB1A8v(pTM100), and JB1A8v(pJB69). Whole-cell extracts were fractionated by SDS-PAGE and transferred to nitrocellulose membranes. Membranes were probed with a rabbit polyclonal anti-invasin antibody as described in Materials and Methods. The arrow points to the band representing full-length invasin. It is typical to see full-length invasin along with several breakdown products. (B) Invasion phenotype and AP activity exhibited by JB41v(pTM100), JB1A8v(pTM100), and JB1A8v(pJB69). AP activities were assayed in duplicate and are represented as means ± ranges. Invasion assays with HEp-2 cells were performed in duplicate, and data represent means ± ranges as described in Materials and Methods. (C) Motility assays were performed with JB41v(pTM100), JB1A8v(pTM100), and JB1A8v(pJB69) in 0.3% motility soft agar plates incubated at 23°C for 16 h. (D) Western analysis of flagellin preparations probed with monoclonal antibody 15D8 as described in Materials and Methods. Data shown are from a single experiment and are representative of several experiments performed with similar results.