FIG. 4.

Invasin and motility phenotypes of the clpB mutant JB69Qv. (A) Whole-cell extracts of indicated strains grown at 23°C were fractionated by SDS-PAGE and transferred to nitrocellulose membranes. Membranes were probed with a rabbit polyclonal anti-invasin antibody as described in Materials and Methods. The arrow points to the band representing full-length invasin. (B) Motility assays were performed with JB580v and JB69Qv in 0.3% motility soft agar plates incubated at 23°C for 16 h. (C) Western analysis of flagellin preparations probed with monoclonal antibody 15D8 as described in Materials and Methods. Arrows point to the three flagellin subunits in Y. enterocolitica. Data shown are from a single experiment and are representative of several experiments performed with similar results.