TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Y. enterocolitica | ||
| 8081v | Serogroup O:8; pYV8081 Nalr | R. Martinez |
| JB580v | Derivative of 8081v; R− M+ Nalr | 22 |
| JB41v | JB580v with wild-type inv; inv::phoA; Nalr Cmr | 1 |
| JB1A8v | JB580v uvrC::mTn5Km; regulatory mutant; Nalr Cmr Kmr | 1 |
| JB69Qv | JB580v clpB::TnMax2; Nalr Ermr | This work |
| BY1v | JB580v clpB::pEP185.2; Nalr Cmr | This work |
| E. coli | ||
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-TC::Mu; Kmr | 46 |
| DH5α | supE44 [lacU169(φ80dlacZΔM15)] hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 15 |
| E131 | F− φ(80dlacZΔM15)Δ(lacZYA argF)U169 deoR recA1 endA1 hsdR17 supE44 λ-thi-1 gyrA96 relA1λ-CH616 | 14 |
| LE392 | F−hsdR514 supE44 supF58 | 25 |
| Plasmids | ||
| pLAFR2 | Mobilizable cosmid vector; Tetr | S. Libby |
| pJQ200KS | Mobilizable suicide vector; Gmr | 41 |
| pTM100 | Mobilizable derivative of pACYC184; Tetr Cmr | 27 |
| pCR2.1 | PCR product cloning vector; Ampr Kmr | Invitrogen |
| pEP185.2 | Mobilizable suicide vector; Cmr | 22 |
| pRK2013 | ColE1 Tra (RK2)+; Kmr | 10 |
| pUC19 | Cloning vector; Apr | New England Biolabs |
| pTnMax2 | TnMax2 delivery plasmid; Ermr | 14 |
| pWSK129 and pWSK130 | Low-copy-number cloning vectors with pSC101 ori; Kmr | 51 |
| pJB32 | pLAFR2 containing an ∼25-kb insert; Tetr | This work |
| pJB69 | pTM100 containing a 5.4-kb EcoRI fragment from pJB32 carrying clpB; Tetr | This work |
| pJB69-Q | pJB69 clpB::TnMax2; Tetr Ermr | This work |
| pCRII-Q | pCR2.1 containing clpB::TnMax2 PCR product; Apr Kmr Ermr | This work |
| pEP185.2-Q | pEP185.2 containing clpB::TnMax2 PCR product; Cmr Ermr | This work |
| pJB302 | pCR2.1 containing clpB PCR product; Apr Kmr | This work |
| pJB305 | pTM100 containing clpB PCR product; Tetr | This work |
| pAVKF | pACY184 containing a 2.2-kb EcoRV fragment carrying fliA and upstream sequences; Cmr | 21 |
| pVM112 | pMT11HC containing inv; Apr | 36 |
| pKVS2 | pBluescript containing a 2.2-kb SacII fragment of the 3′ end of fleA, all of fleB, and the 5′ end of fleC; Apr | 20 |
| pBY38 | pEP185.2 with a 744-bp SalI-SacII fragment from pAJD70 | This work |
| pAJD69 | pWSK129 containing a 3.6-kb EcoRI-PstI fragment of pAJD11 carrying clpB | This work |
| pAJD70 | pWSK130 containing a 3.6-kb EcoRI-PstI fragment of pAJD11 carrying clpB | This work |
| pAJD11 | pWSK129 containing a 5.4-kb EcoRI fragment carrying clpB; Kmr | This work |