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. 2024 Apr 30;18(19):12168–12186. doi: 10.1021/acsnano.3c12539

Table 1. Image Acquisition Widefield Fluorescence Inverted Microscope (Olympus).

dye light source excitation filter (nm) emission filter (nm) exposure time (ms) power (%) objective
HXT 33342 405 nm 391–437 420–460 2 50 UPlanFL N 40×/0.6 air
Sytox Green 470 nm 460–495 510–550 10 50 UPlanFL N 40×/0.6 air
MMIR1 (reference) 580 nm 545–580 610 nm long-pass 20 25 UPlanFL N 40×/0.6 air
MMIR1 (O2 sensitive) 635 nm none/685 nm long-pass 705–845 nm 20 25 UPlanFL N 40×/0.6 air
Calcein Green-AM 490 nm 460–495 510–550 20 50 UPlanFL N 40×/0.6 air
Propidium iodide (PI) 550 nm 545–580 610 nm long-pass 25 25 UPlanFL N 40×/0.6 air
TMRM 550 nm 545–580 610 nm long-pass 5 25 UPlanFL N 20×/0.45
NADPH113 460 nm 460–495 510–550 120 25 UPlanFL N 40×/0.6 air
FAD 460 nm 460–495 510–550 40 50 UPlanFL N 40×/0.6 air