Table 1. Image Acquisition Widefield Fluorescence Inverted Microscope (Olympus).
dye | light source | excitation filter (nm) | emission filter (nm) | exposure time (ms) | power (%) | objective |
---|---|---|---|---|---|---|
HXT 33342 | 405 nm | 391–437 | 420–460 | 2 | 50 | UPlanFL N 40×/0.6 air |
Sytox Green | 470 nm | 460–495 | 510–550 | 10 | 50 | UPlanFL N 40×/0.6 air |
MMIR1 (reference) | 580 nm | 545–580 | 610 nm long-pass | 20 | 25 | UPlanFL N 40×/0.6 air |
MMIR1 (O2 sensitive) | 635 nm | none/685 nm long-pass | 705–845 nm | 20 | 25 | UPlanFL N 40×/0.6 air |
Calcein Green-AM | 490 nm | 460–495 | 510–550 | 20 | 50 | UPlanFL N 40×/0.6 air |
Propidium iodide (PI) | 550 nm | 545–580 | 610 nm long-pass | 25 | 25 | UPlanFL N 40×/0.6 air |
TMRM | 550 nm | 545–580 | 610 nm long-pass | 5 | 25 | UPlanFL N 20×/0.45 |
NADPH113 | 460 nm | 460–495 | 510–550 | 120 | 25 | UPlanFL N 40×/0.6 air |
FAD | 460 nm | 460–495 | 510–550 | 40 | 50 | UPlanFL N 40×/0.6 air |