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. 2024 Mar 11;7:0335. doi: 10.34133/research.0335

Fig. 4.

Fig. 4.

HBO inhibits glycolysis and enhances OXPHOS in Panc02 CSCs. (A) KEGG-enriched pathway analysis based on metabolite differences between hypoxia and HBO. (B) Up- and down-regulation of metabolites in volcano plots of hypoxia versus HBO based on targeted metabolomic analysis (n = 6, FC ≥ 2, VIP ≥ 1). (C) Decreased metabolites in the HBO group compared to the hypoxia group in the glycolytic pathway. Levels of 2-NBDG uptake (D), intracellular LA (E), and intracellular pyruvate (F) of Panc02 CSCs under hypoxia, HBO, and normoxia by flow cytometry (mean ± SEM, n = 3). (G) WB analysis of Panc02 CSCs treated with hypoxia, HBO, and normoxia for 24 h. (H) OCR of Panc02 CSCs under hypoxia and HBO (mean ± SEM, n = 4). (I) Levels of intracellular MitoROS under hypoxia, HBO, and normoxia by flow cytometry (mean ± SEM, n = 3). (J) Measurement of oxygen consumption of Panc02 CSCs under different treatment using a Clark oxygen electrode. (K) Quantification of Panc02 CSCs OCRs (mean ± SEM, n = 3). Statistical significance was calculated by t test. *P < 0.05, **P < 0.01, ***P < 0.001.