KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti-EGFP | Columbia University | RRID: AB_3094577 |
| Rabbit anti-dsRed | Columbia University | RRID: AB_3086780 |
| Guinea pig anti-vGlut1 | Columbia University | RRID: AB_2665455 |
| Guinea pig anti-vGlut2 | Millipore Sigma | RRID: AB_2665454 |
| Donkey anti-chicken Alexa Fluor 488 | Jackson ImmunoResearch | RRID: AB_2340375 |
| Donkey anti-rabbit Cy3 | Jackson ImmunoResearch | RRID: AB_2307443 |
| Donkey anti-guinea pig DyLight 405 | Jackson ImmunoResearch | RRID: AB_2340470 |
| NeuroTrace 640/660 | ThermoFisher | RRID: AB_2572212 |
| Bacterial and virus strains | ||
| AAV-retro-CAG-tdTomato | Addgene | Cat# 59462-AAVrg |
| AAV1-hSyn-Cre-WPRE.hGh | Addgene | Cat# 105553-AAV1 |
| AAV1-EF1a-Flpo | Addgene | Cat# 55637-AAV1 |
| AAV-retro-hSyn-Cre-WPRE.hGh | Addgene | Cat# 105553-AAVrg |
| AAV-retro-EF1a-Flpo | Addgene | Cat# 55637-AAVrg |
| AAV2-hSyn-ChR2-EYFP-WPRE.hGH | UNC Vector Core | N/A |
| AAV-DJ-FLEX-H2B-GFP | Columbia University | N/A |
| AAV-hSyn-FlpX-H2B-dTomato | Columbia University | N/A |
| AAV6-CAG-FLEX-N2cG-mKate2.0 | Janelia Farm | N/A |
| AAV1-EF1a-FLEX-TVA-mCherry | UNC Vector Core | N/A |
| AAV2/1-CAG-FRT-TVB | Janelia Farm | N/A |
| AAV1-CAG-FLEX-GFP | UNC Vector Core | N/A |
| AAV1-EF1a-fDIO-tdTomato | Addgene | Cat# 128434-AAV1 |
| EnVA-N2c-deltaG-GFP | Thomas Jefferson | N/A |
| EnVB-N2c-deltaG-tdTomato | Janelia Farm | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Cholera Toxin Subunit Alexa Fluor 488 Conjugate | ThermoFisher | Cat# C34775 |
| Cholera Toxin Subunit Alexa Fluor 555 Conjugate | ThermoFisher | Cat# C34776 |
| Tetrodotoxin citrate | Tocris | Cat# 1069 |
| 4-Aminopyridine | Tocris | Cat# 0940 |
| TrueBlack | Biotium | Cat# 23007 |
| Deposited data | ||
| Single nucleus RNA sequencing of spinal neurons targeted by CFA or RFA | This study | NIH BioProject: PRJNA1081900 |
| Code for analysis of anatomical data | This study | Zenodo: https://doi.org/10.5281/zenodo.10712498 |
| Experimental models: Organisms/strains | ||
| C57BL/6J mice | The Jackson Laboratory | IMSR_JAX:000664 |
| B6; 129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J | The Jackson Laboratory | IMSR_JAX:021039 |
| Software and algorithms | ||
| MATLAB | Mathworks | RRID:SCR_001622 |
| R | The R Project for Statistical Computing | RRID:SCR_001905 |
| Seurat | Hao et al. 202175 | RRID:SCR_016341 |
| BrainJ | Botta et al. 202024 | N/A |
| SpinalJ | Fiederling et al. 202127 | N/A |
| Prism | GraphPad | RRID:SCR_002798 |