KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Dsg3 antibody | Abcam | Cat # ab231309; RRID: N/A |
| E-cadherin antibody | Abcam | Cat # ab231303; RRID: AB 2923285 |
| Alexa Fluor 647 | Thermo Fisher | Cat # A-21235; RRID: N/A |
| Alexa Fluor 488 | Thermo Fisher | Cat # A-11001; RRID: N/A |
| Alexa Fluor 488 Phalloidin | Thermo Fisher | Cat # A12379; RRID: N/A |
| Anti-Dsg3 antibody (AK23) | MBL | Cat # D218-3; RRID: AB_591236 |
| PX4-4 | This study | N/A |
| PX4-3 | This study | N/A |
| Anti-TPO | Novus Biologicals | Cat # NBP1-50811; RRID: N/A |
| AtS13 | This study | N/A |
| PVIgG | This study | N/A |
| rabbit anti-GFP | Abcam | Cat # ab6556; RRID: AB_305564 |
| 6 nm colloidal gold conjugated goat-anti-rabbit IgG | Electron Microscopy Sciences | Cat # 25103; RRID: N/A |
| rabbit anti-GFP | Cell Signaling | Cat # 2956S; RRID: N/A |
| mouse anti-desmoplakin | Fitzgerald | Cat # 10R-D108a; RRID: AB 1284113 |
| mouse anti-E-cadherin | BD Biosciences | Cat # 610181; RRID: AB 397580 |
| anti-active RhoA GTP | NewEast Biosciences | Cat # 26904; RRID: AB_1961799 |
| EGTA | Biotechne | Cat # 2807; RRID: N/A |
| rabbit anti-RhoA | Cell Signaling | Cat # 2117S; RRID: N/A |
| mouse anti-GAPDH | Santa Cruz Biotechnology | Cat # SC-32233; RRID: AB_627679 |
| HRP-linked anti-mouse IgG | Jackson Immunoresearch | Cat # 115-035-166; RRID: AB 2338511 |
| HRP-linked anti-rabbit IgG | Jackson Immunoresearch | Cat # 111-035-003; RRID: AB 2313567 |
| Chemicals, peptides, and recombinant proteins | ||
| Dsg3 tension sensor | This study | N/A |
| Dsg3 tailless control sensor | This study | N/A |
| pTriEx-RhoA FRET WT biosensor | Addgene | Plasmid #12150; RRID: Addgene_12150 |
| LZBob-neo-vector | Gift from James K. Wahl, III | N/A |
| ROCK Inhibitor (Y27632) | Sigma-Aldrich | Cat # SCM075 |
| Y27632 | Sellect Chemical LLC | Cat # S1049 |
| Rho activator | Cytoskeleton | Cat # CN01-A |
| Polybrene | Sigma | Cat # 28728-55-4 |
| G418 | Invivogen | Cat # ant-gn-1 |
| Aurion Blocking Solution | Electron Microscopy Sciences | Cat # 25595 |
| BSA | Thermo Fisher Scientific | Cat # 37520 |
| glycine | Millipore Sigma | Cat # 410225 |
| Tween 20 | Millipore Sigma | Cat # P7949 |
| protease-inhibitor cocktail | Sigma Aldrich | Cat # S8830 |
| protein assay dye reagent | Bio-Rad | Cat # 5000006 |
| ECL chemiluminescence | Santa Cruz Biotechnology | Cat # sc-2048 |
| Critical commercial assays | ||
| pAdEasy adenoviral vector system | Agilent | Cat # 240009 |
| MBL (MBL2) Human ELISA Kit | ThermoFisher | Cat # EHMBL2 |
| Zeba Spin Desalting Columns | ThermoFisher | Cat # 89882 |
| Pierce Centrifuge column | ThermoFisher | Cat # 89896 |
| Melon Gel IgG Spin Purification Kit | ThermoFisher | Cat # 45206 |
| Gel with 0.2 μm fluorescent beads | Matrigen | Softview 35/20 Glass |
| Experimental Models: Cell Lines | ||
| HaCaT cells | Gift from James K. Wahl, III | N/A |
| 293A cells | ThermoFisher | Cat # R70507 |
| Phoenix cells | Gift from James K. Wahl, III | N/A |
| Recombinant DNA | ||
| E-cadherin-GFP | Addgene | Plasmid # 28009; RRID: Addgene_28009 |
| Software and algorithms | ||
| MATLAB R2020b | MathWorks | https://www.mathworks.com/products/new products/release202 0b.html |
| Fiji | NIH | https://imagei.net/software/fiii/ |
| GraphPad Prism | GraphPad Prism 9 | https://www.graphpad.com/updates/prism-900-release-notes |
| Zen | Zeiss | https://www.micro-shop.zeiss.com/en/de/softwarefinder/software-categories/zen-blue/zen-blue-pro |
| LASX Office | Lecia | https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/downloads/ |
| ImageLab Software | Bio-Rad | https://www.bio-rad.com/en-us/sku/12012931-image-lab-software-usb-drive?ID=12012931 |
| Other | ||
| Confocal microscope | Zeiss | LSM800 |
| Inverted Fluorescent microscope | Leica | DMi8 |
| Leica EM UC6i ultramicrotome | Leica | EM UC6i |
| Transmission electron microscope | Jeol | JEM-1230 |
| 3D Printer | Stratasys | Objet500 Connex3 |
| Nanodrop® spectrophotometer | Thermo Scientific | Cat # ND2000CLAPTOP |
| ChemiDoc imager | Bio-Rad | https://www.bio-rad.com/en-us/category/chemidoc-imaging-systems?ID=NINJ0Z15 |
| PTFE resin molds | Ted Pella, Inc. | Cat # 10509 |
| Aclar film | Ted Pella, Inc. | Cat # 10501 |
| polyvinylidene fluoride (PVDF) membranes | Bio-Rad | Cat # 1620177 |