Figure 1.

Morphological profiling using the Cell Painting assay. a) Schematic representation of Cell Painting assay; cells are incubated and perturbed and a set of six stains is applied. Images are then obtained by automated microscopy followed by nucleus and cell body segmentation. b) Appropriate software or deep learning-based methods are applied to measure or calculate morphological features from the images. c) After feature pre-processing, downstream analysis is performed. This includes a variety of methods, including supervised and unsupervised machine learning, to better elucidate the biological effects of a compound, such as its MoA or safety profile.