Key Resources
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Chemicals | ||
| Fasnall benzenesulfonate salt | MilliporeSigma | SML1815 |
| 1-benzyl-N-{5,6-dimethylthieno[2,3-d]pyrimidin-4-yl}pyrrolidin-3-amine (Fasnall) | Enamine | EN300-14327544 |
| 1-benzyl-N-{5,6-dimethylthieno[2,3d]pyrimidin-4-yl}pyrrolidin-3-amine hydrochloride (Fasnall) | Enamine | EN300-45430166 |
| GSK2194069 | Tocris | 5303 |
| Cerulenin | MilliporeSigma | C2389 |
| C75 | Tocris | 2489 |
| TVB-2640 | Selleck Chem | S9714 |
| TVB-3166 | Selleck Chem | S3576 |
| Methanol, Optima™ LC/MS Grade, Fisher Chemical™ | Fisher Scientific | A456 |
| Acetonitrile, Optima™ LC/MS Grade, Fisher Chemical™ | Fisher Scientific | A955 |
| Water, Optima™ LC/MS Grade, Fisher Chemical™ | Fisher Scientific | W6 |
| Isopropanol, Optima™ LC/MS Grade, Fisher Chemical™ | Fisher Scientific | A461 |
| Malonic acid | MilliporeSigma | M1296 |
| Succinic acid | MilliporeSigma | S3674 |
| Acetyl-CoA | MilliporeSigma | A2056 |
| Malonyl-CoA | MilliporeSigma | M4263 |
| Malonyl-L-carnitine | MilliporeSigma | 07441 |
| Dimethyl malonate | MilliporeSigma | 136441 |
| [U-13C6] D-glucose | Cambridge Isotope Laboratories | CLM-1396 |
| [U-13C5] L-glutamine | Cambridge Isotope Laboratories | CLM-1822-H |
| [13C2] Acetate | Cambridge Isotope Laboratories | CLM-440 |
| Deuterium oxide, for NMR, 99.8 atom % D | Thermo Fisher Scientific | 166301000 |
| Corning® 1L RPMI 1640 | Corning | 10-040-CM |
| Corning® 100 mL Trypsin EDTA 1X | Corning | 25-053-CI |
| Corning® 100 mL Penicillin-Streptomycin Solution, 100x | Corning | 30-002-CI |
| Phosphate buffered saline | MilliporeSigma | P3813 |
| RPMI-1640 Medium, with L-glutamine, without sodium bicarbonate, powder | MilliporeSigma | R6504 |
| Gibco RPMI 1640 Medium, no glucose | Thermo Fisher Scientific | 11879020 |
| Ammonium sulfate, ultrapure | Thermo Fisher Scientific | J6441922 |
| L-(-)-Malic acid | Thermo Fisher Scientific | 150590250 |
| Oxaloacetic acid | MilliporeSigma | O4126 |
| NADH | Roche | 10128023001 |
| NAD+ | Roche/MilliporeSigma | NAD100-RO |
| DMSO | Thermo Fisher Scientific | J66650AD |
| Saponin | MilliporeSigma | 84510 |
| Oligomycin from Streptomyces diastatochromogenes | MilliporeSigma | O4876 |
| CCCP | MilliporeSigma | C2759 |
| Rotenone | MilliporeSigma | R8875 |
| Antimycin A from Streptomyces sp. | MilliporeSigma | A8674 |
| Potassium cyanide | Thermo Fisher Scientific | L1327322 |
| LW6 | Tocris | 6322 |
| Metformin | MilliporeSigma | 317240 |
| (R,R)-Chloramphenicol | Thermo Fisher Scientific | B20841.14 |
| R&D Systems™ Cultrex Basement Membrane Extract, Type 3, Pathclear | R&D Systems | 363201002 |
| Ethiqa XR (buprenorphine) ExtendedRelease Injectable Suspension, 1.3mg/mL, C3 | Fidelis Animal Health | FP-001 |
| 17β-Estradiol 0.5 mg/p 60 days 50 per lot | Innovative Research of America | SE-121 |
| Biological Samples | ||
| Fetal Bovine Serum, Dialyzed, 0.1 um sterile-filtered | Gemini Bio | 100–108 |
| L-Malate Dehydrogenase (L-MDH) from pig heart (mitochondrial) | Roche/MilliporeSigma | LMDH-RO |
| Experimental Models: Cell Lines | ||
| BT-474 | ATCC | HTB-20 |
| MDA-MB-468 | ATCC | HTB-132 |
| SUM159PT | BioIVT (Asterand) | SUM159PT |
| MCF7 | ATCC | HTB-22 |
| HCC1806 | ATCC | CRL-2335 |
| MDA-MB-231 | ATCC | CRM-HTB-26 |
| HS578T | ATCC | HTB-126 |
| CAL120 | DSMZ | ACC 459 |
| OMM1.3 | A gift from Dr. Andrew Aplin, Thomas Jefferson University (Philadelphia, PA, USA) | |
| Experimental Models: Patient-derived Xenografts | ||
| WM3939 (WM3939-2), BRAFi/MEKi resistant melanoma | Dr. Meenhard Herlyn’s Lab, The Wistar Institute | |
| WM4336 (WM4336-5), BRAFi/MEKi resistant melanoma | Dr. Meenhard Herlyn’s Lab, The Wistar Institute | |
| Experimental Models: Mouse strains | ||
| NOD-scid IL2Rγnull (NSG) | The Wistar Institute Animal Facility | |
| Commercial Assays | ||
| Mycoplasma PCR Detection Kit | abm | G238 |
| PDH Activity Assay Kit | MilliporeSigma | MAK183 |
| Seahorse XF Cell Mito Stress Test Kit for Seahorse XF96 | Agilent Technologies | 103015-100 |
| BIOLOG MitoPlate S-1 | BIOLOG | 14105 |
| MitoCheck® Complex I Activity Assay Kit | Cayman Chemical | 700930 |
| Complex I Enzyme Activity Assay Kit (Colorimetric) | Abcam | ab109721 |
| Deposited Data | ||
| All LC-MS and LC-MS/MS data | Metabolomics Workbench |
https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Project&ProjectID=PR001941
http://dx.doi.org/10.21228/M8TM76 |
| Software and Algorithms | ||
| R | The R Project for Statistical Computing https://www.r-project.org/ |
4.3.1 |
| RStudio | Posit Software, PBC https://posit.co/download/rstudiodesktop/ |
2023.12.1 |
| ProteoWizard | https://proteowizard.sourceforge.io | 3.0.20365 |
| MAVEN | Rabinowitz Lab, Lewis-Sigler Institute for Integrative Genomics Princeton University, New Jersey, USA https://1drv.ms/u/s!AjmvFyh3ByMggYeFP3vyplLyh2rDlUs?e=BbOhNe |
6.82 |
| XCalibur | Thermo Fisher Scientific, Inc. | 4.2.47 |
| Analyst | AB SCIEX LLC | 1.7.3 HotFix 1 |
| MultiQuant | AB SCIEX LLC | 3.0 HotFix 4 |
| Biorender | https://www.biorender.com | |
| Adobe Illustrator | Adobe, Inc. | 28.2 |
| MetaboAnalyst | Xia Lab, Department of Microbiology and Immunology, McGill University (Montreal, Quebec, Canada) https://www.metaboanalyst.ca |
6.0 |
| Fiji | https://fiji.sc | |