Figure 5: Single dose DT administration allows for the assessment of ASC reconstitution kinetics in J-DTR mice. Related to Figure S4.

(A) Schematic showing DT treatment of J-DTR mice. 3–4 months old animals were given a single i.p. dose of 200 ng DT in 100 μL 1x PBS. Control mice received 100 μL of 1x PBS. Mice were euthanized at days 1, 3 and 7 post-injection. SPL, BM and THY were assessed for ASCs via flow cytometry. Schematic made with BioRender. (B-D) Total cell numbers for (B) SPL, (C) BM and (D) THY of J-DTR mice treated with PBS or DT. Data presented on Log₁₀ scale to show full range. (E-G) Total ASC numbers for (E) SPL, (F) BM and (G) THY of J-DTR mice treated with PBS or DT. Data presented on Log₁₀ scale to show full range. (H-J) DTR gMFIs for ASCs from (H) SPL, (I) BM and (J) THY of J-DTR mice treated with PBS or DT. (B-J) Symbols represent individual female (orange) and male (blue) mice. Horizontal lines represent mean ± SEM. J-DTR day 1 PBS: female n = 2, male n = 2; J-DTR day 1 DT: female n = 2, male n = 1; J-DTR day 3 PBS: female n = 2, male n = 2; J-DTR day 3 DT: female n = 1, male n = 2; J-DTR day 7 PBS: female n = 2, male n = 2; J-DTR day 7 DT: female n = 2, male n = 2. (B-G) Statistics: Kruskal-Wallis test (nonparametric) with Dunn’s multiple comparisons test. Comparisons made between PBS and DT treatments for a given day. (H-J) Statistics: One-way ANOVA with Tukey’s multiple comparisons test. Comparisons made between D1, D3 and D7 for a given treatment.