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qPCR validation of differentially expressed miRNAs (in silico)in Ehrlichia chaffeensis infected and partially fed tick tissues. A) Midgut B) Salivary glands. Expression of miRNAs was normalized with clean and partially fed tick tissues (indicated as 1 on the y-axis). Statistical significance for qRT-PCR-based differential expression was determined by the 2-tailed Student’s t-test where * is p<0.05. At least three biological replicates were used in each of the experiments.
