Figure 7. Dynamic chromatin regions in the stg cis-regulatory region have enhancer activity in vivo.

(A) ATAC-seq accessibility data at the stg locus across tissues and timepoints. Fragments used to direct Gal4 expression in publicly available driver lines are depicted by black bars, each of which was tested for enhancer activity. (B-D) Enhancer reporter expression data are presented for driver lines GMR31F07, GMR32C11, and GMR32F08, which are highlighted by gray boxes in (A). Each driver was tested in wing, eye and/or brain at L3, 24h APF and 44h APF time points. Images show the readout of ‘current’ Gal4 activity (UAS-RFP) and signal intensities are qualitative to emphasize the distinct spatial domains of activity across driver lines. Comprehensive data from all drivers is available in Supp. Figs. 5–7. (E) HCR-FISH data showing stg transcript expression in wing, eye and brain at L3, 20h, 24h, and 44h APF. (F) Line plot showing stg Log2-transformed counts per million (CPM) expression levels via RNA-Seq in wing and eye at L3, 24h and 44h APF timepoints, and in brain at L3 and 48h APF timepoints. Individual data points represent values from RNA-Seq replicates.