Figure 5: Importin mediated HBV capsid interaction with NuPODs.

(A) HBV capsids mixed with capped 60-nm Nup62 NuPODs in the presence of 100 nM importin β1 (impβ). (B) HBV capsids mixed with capped 60-nm Nup153 NuPODs in the presence of 100 nM impβ. (C) HBV capsids mixed with capped 60-nm Nup62-Nup153 NuPODs in the presence of 100 nM impβ. For (A)–(C), schematic diagrams of the binding experiments are shown next to representative TEM images. Scale bar: 100 nm. (D) Percentages of NuPODs occupied by HBV capsids with (green) or without (grey) 100 nM impP. NuPODs counted in each experiment (from left to right): 288, 206, 168, 380, 279 and 203. Occupancies of importin-free Nup153 and Nup62-Nup153 NuPODs (Figure 4E) are shown here for comparison.