TABLE 3.

Expression of acsA-lacZ fusion in ccpA mutants^a

Strain	ccpA allele	β-Galactosidase activity		Ratio
		− Glucose	+ Glucose
BR151MACWT	Wild type	54	2.5	22
CPC18AV	ccpA18AV	46	10	4.6
CPC48RC	ccpA48RC	51	11	4.6
CPC286PL	ccpA286PL	45	9.0	5.0
BR151MAccp::spc	ccpA::spc	54	46	1.2

^aAll strains contained an acsZ-lacZ transcriptional fusion (4). Cells were grown in NSM medium (19) in the absence (− glucose) or presence (+ glucose) of glucose (1%). β-Galactosidase activities are expressed in Miller units (13) and indicate activity 1 h after the time of entry of the culture into stationary phase. The ratio indicates the level of repression during growth in the presence of glucose. All growth experiments were carried out at least twice, and samples were assayed in duplicate; variation was less than 10%.