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Acetate concentrations in cultures of ccpA mutants
| Strain | ccpA allele | Acetate concn (mM)a
|
|
|---|---|---|---|
| − Glucose | + Glucose | ||
| BR151MACWT | Wild type | 0.50 | 13 |
| CPC18AV | ccpA18AV | 0.65 | 9.9 |
| CPC48RC | ccpA48RC | 0.57 | 12 |
| CPC286PL | ccpA286PL | 0.65 | 11 |
| BR151MAccp::spc | ccpA::spc | 0.75 | 7.3 |
Cells were grown in TSS medium containing 1% Casamino Acids (− glucose) or containing 1% Casamino Acids and 1% glucose (+ glucose). Samples were taken 30 min prior to the time of entry of the culture into stationary phase.
