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. Author manuscript; available in PMC: 2024 May 17.
Published in final edited form as: J Vis Exp. 2021 Nov 10;(177):10.3791/63253. doi: 10.3791/63253

Figure 1: Schematic for isolation of murine lung endothelial cells.

Figure 1:

Lungs from 5–7 day old neonatal pups are harvested and minced. Minced tissue was enzymatically digested with collagenase I. Cellular suspension is plated and cultured for 2–3 days. Cells then undergo primary immunobead isolation with CD-31-coated beads and are cultured for another 3–4 days. Second immunobead isolation with CD-102-coated beads before culturing another 2–3 days. Cells are now ready for functional experimentation. The image was created by a web-based illustration tool, Biorender.