Materials
| Name | Company | Catalog Number | Comments |
|---|---|---|---|
| 50 mL conicals | Corning Life Sciences | 430829 | |
| Accutase Cell Detachment Solution | Innovative Cell Technologies, Inc | AT-104 | |
| BD PrecisionGlide Needle 25 G x 5/8” | Becton Disckinson | 305122 | |
| BioRender.com | BioRender | ||
| Blunt Cannula 15 G x 1-1/2” | Covidien | 8881202314 | |
| CD-102 Rat anti-mouse (3C4 (MIC2/4)) | BD Biosciences | 553326 | |
| CD-31 Rat anti-mouse (MEC 13.3) | BD Biosciences | 557355 | |
| Collagenase, Type 1 | Worthington Biochemical Corp | LS004194 | |
| DMEM, high glucose | Gibco | 11965092 | |
| Dynabeads Sheep Anti-Rat IgG | Invitrogen | 11035 | |
| Endothelial Cell Growth Supplment | EMD Millipore Corp | 02-102 | |
| Falcon cell strainers (70 µm) | Corning Life Sciences | 352350 | |
| Fetal Bovine Serum, heat inactivated | Gibco | 10082-147 | |
| Fine Scissors | Fine Science Tools | 14060-09 | |
| Fine Scissors | Fine Science Tools | 14060-10 | |
| Fisherbrand Disposable Borosilicate Glass Pasteur Pipets | Fisher Scientific | 13-678-20B | |
| FITC Rat Anti-mouse CD102 (3C4) | BD Biosciences | 557444 | |
| FITC Rat IgG2a, κ Isotype Control (R35-95) | BD Biosciences | 553929 | |
| Graefe Forceps | Fine Science Tools | 11050-10 | |
| HBSS, no calcium, no magnesium, no phenol red | Gibco | 14175095 | |
| Heparin Sodium Injections (1,000 Units/mL) | Medline | 0409-2720-02 | |
| Heparin sodium salt from porcine intestinal mucosa | Sigma | H3393 | |
| HEPES (1 M) | Gibco | 15630106 | |
| Nonessential amino acids (100x) | Gibco | 11140050 | |
| PE Rat Anti-mouse CD31 (MEC 13.3) | BD Biosciences | 553373 | |
| PE Rat IgG2a, κ Isotype Control R35-95) | BD Biosciences | 553930 | |
| Penicillin Streptomycin (10,000 U/mL, 10,000 µg/mL) | Gibco | 15140122 | |
| Recombinant Murine IFN-γ | Peprotech | 315-05 | |
| Recombinant Murine IL-1β | Peprotech | 211-11B | |
| Recombinant Murine TNF-α | Peprotech | 315-01A | |
| Round bottom polystyrene test tubes | Corning Life Sciences | 352058 | |
| Semken Forceps | Fine Science Tools | 11008-13 | |
| Sodium pyruvate (100 mM) | Gibco | 11360070 | |
| Stericup Quick Release-GP Sterile Vacuum Filtration System, 250 mL | Millipore | S2GPU02RE | |
| Steriflip-GP Sterile Centrifuge Tube Top Filter Unit | Milipore | SCGP00525 | |
| Sterile syringe (10 mL) | Fisher Scientific | 14-955-459 | |
| Sterile syringe (20 mL) | Fisher Scientific | 14-955-460 | |
| T-75 cell culture flask with vent cap, CellBIND treated | Corning Life Sciences | 3290 |