. 2024 May 7;20(5):e1011230. doi: 10.1371/journal.pgen.1011230

Table 1. Summary of FECD case and control-derived corneal endothelial cell (CEC) cultures analysed by short and long-read RNA-seq, and spatial transcriptomics.

Sample	Sex	Age^*	Ethnicity^a/Ancestry^b
Long-read
Control 1	Female	68	White-European^a
Control 2	Male	64	White-European^a
Control 3	Female	74	White-European^a
Control 4	Male	69	White-European^a
FECD1exp+ (43/90)	Female	74	White-European^a^,^b
FECD2exp+ (14/80)	Male	78	White-European^a ^,b
FECD3exp+ (18/86)	Female	70	White-European^a^,^b
FECD4exp+ (26/78)	Female	45	White-European^b
Short-read
Control 1	Female	68	White-European^a
Control 5	Male	55	White-European^a
Control 6	Male	55	White-European^a
Control 7	Female	56	Black-African^a
FECD5exp+ (16/86)	Female	67	White-European^a^,^b
FECD6exp+ (12/106)	Male	69	White-European^a^,^b
FECD7exp+ (16/94)	Female	54	White-European^a^,^b
FECD1exp- (13/18)	Female	64	European/Black-Carribbean^a Black-African^b
FECD2exp- (18/24)	Female	73	White-European^a^,^b
FECD3exp- (22/28)	Male	53	Black-African^a^,^b
Spatial transcriptomics
Control 8 (12/16)	Female	45	Black-African^a
Control 9 (15/33)	Female	46	White-European^a
Control 10 (25/26)	Male	70	White-European^a
FECD4exp- (15/15)	Female	48	White-European^a
FECD5exp- (16/23)	Female	57	Black-African^a
FECD6exp- (15/16)	Female	58	Black-African^a
FECD8exp+ (63/88)	Female	75	White-European^a
FECD9exp+ (12/76)	Male	61	Black-African^a
FECD10exp+ (18/67)	Male	65	White-European^a

CTG18.1 expansion status is denoted in brackets for all FECD-case derived CEC lines analysed.

*Age at time of corneal tissue excision.

^a Self-reported by affected individual.

^b Ancestry determined by applying a principal component analysis (PCA) method, FRAPOSA [22]. All control samples were derived from donor corneas with very high CEC counts (≥2,600 cells/mm2) and endothelium free of guttae or any other abnormalities.