Fig. 2.

Alignment and phylogenetic tree of the PL24 ulvan lyase isolated from Vibrio sp. FNV38. PL24 ulvan lyase isolated from Vibrio sp. FNV38 is represented as ‘VUL’ in the tree; the rest of lyase are labelled following CAZy DB annotation (http://www.cazy.org/PL24_characterized.html). A. Alignment of ulvan lyase sequences. Each position of the alignment is coloured according to the conservation from dark blue (conserved) to white (non-conserved). The conservation is also shown as a bar plot in the bottom of the alignment. Red “*” marks indicate catalytic residues as described in (Xu et al. 2021). B. Phylogenetic tree; the vertical red line define the distance (as computed using BLOSUM62) separating the two major groups on the PL24 family. Values at the branching points indicate the bootstrap values (out of 50 rounds)