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. 2024 May 17;15:3812. doi: 10.1038/s41467-024-48088-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Schematic image of ELASTomics with CITE-seq for mHSPCs. c-kit⁺ bone marrow (BM) cells were isolated from a C57BL/6 J mouse femur using antibody-conjugated magnetic beads. After DTDs were imported into the c-kit⁺ BM cells by nanopore-electroporation, the cells were incubated with antibodies conjugated with antibody-derived tag (ADT). The DTDs, ADTs, and mRNA expression were measured by scRNA-seq. b–e UMAP of mHSPCs. The color and color gradient indicates the identified cell types (Gra: granulocyte n = 5786; GMP: granulocyte-macrophage progenitor n = 857; Mono: monocyte n = 730; CLP: common lymphoid progenitor n = 113; MPP: multipotential progenitors n = 394; HSC: hematopoietic stem cell n = 131; MkP: megakaryocyte progenitors n = 126; BaP: basophil progenitors n = 69; CMP: common myeloid progenitor n = 206; EryP/MEP, erythroid progenitors / megakaryocyte-erythrocyte progenitors n = 573; Erythroid n = 463; Erythrocyte n = 115; Bcell n = 356; other n = 92) (b), Cd48 gene expression (c), centered log ratios of Cd48 ADT (d), and centered log ratio of 4 kDa DTD (e), respectively. f–i Comparison of the erythroid lineage cells (HSC, MPP, CMP, MEP/EryP, erythroid, and erythrocyte) with centered log ratios of 4 kDa DTD f, centered log ratios of Cd48 ADT (g), percent fractions of mitochondrial mRNA (h), and gene expressions of Sptb (i), respectively. f The percentage indicates the ratio of cells higher than 1 of the centered log ratio of the 4 kDa DTD.