Fig. 4.

Stimulation of NK cells by DENV-infected DCs.

DCs were treated with mock or infected with DENV (MOI 1) for overnight, and the infection was detected intracellularly by anti-dengue antibodies. (A) The representative FACS profile of dengue infected DCs. Cells were gated based on FSC-A and SSC-A, followed by CD14 and DC-SIGN expression. Dendritic cells (CD14⁻DC-SIGN⁺) were gated and analyzed for DENV NS3 antigen expression. Histogram demonstrates frequencies of DENV infected DCs as shown by positive staining with dengue NS3 protein specific mAbs. (B) The representative FACS profile of NK cells (CD56⁺CD3⁻) upon stimulation with mock or dengue infected DCs for 24 h. The frequencies of IFN-γ⁺, TNF-α⁺ and CD107a⁺ NK cells are demonstrated.